Definition: 1) Pass: All gains within 100% baseline and all RCVs <6% for all detectors 2) Caution: All Gains passed, but at least one detector had a RCV above the >6% cutoff. Instrument remains usable but decreased resolution for fluorophores on that detector is possible. 3) Fail: A gain exceeded 100% baseline, or RCVs exceeded >6% for at least the indicator detectors. Significant batch effects may occcur